Sequencing

Our team of experienced technicians can provide support and advice for a wide-range of techniques. We provide both short-read sequencing platforms (Illumina) and long-read sequencing platforms (Oxford Nanopore Technologies).

For more information on sequencing and initial quotes please contact Angie Fawkes or Lee Murphy

To contact the sequencing team for ongoing projects - sample submission, project updates etc - please use the generic sequencing email. This will go to all of our sequencing team (Audrey, Dickie, Louise & Tammy) to ensure the fastest response.

 

A picture of the two Illumina Nextseq2000 and iSeq platforms

Applications

The most difficult part of sequencing is library preparation. Our team has experience in a wide range of techniques to ensure the best quality libraries are produced. We can provide advice on the best methods to use based on your experimental need and sample quality. Some of our most popular applications include:

DNA sequencing

  • Whole genome
  • Exomes
  • Panels

    Epigenetics

    • RRBS
    • ChIP-seq

    Transcriptomics (see poster below for RNA-seq options based on sample quality)

    • RNA-seq
    • miRNA-seq
    • CAGE-seq

     

    Pre-prepared Libraries

    We can sequence your pre-prepared libraries. This is a popular option for single cell and spatial transcriptomics. 

    Platforms

    Short-read sequencing

    Our short-read sequencing is supported by two Illumina NextSeq2000 sequencing platforms and a smaller Illumina iSeq platform. This allows us to offer a flexible and fast service. By changing the flowcell we can change the number of reads sequenced and by changing the cartridge we can change the number of cycles achieved. 

    A table of the output of the Illumina iSeq and NextSeq platforms

    Long-read sequencing

    Our long-read sequencing is supported by Oxford nanopore Technologies PromethION and MinION platforms. The PromethION-24 is for our large scale sequencing and our MinION for smaller throughput, as well as checking library quality before running on the larger PromethION.

    Sequencing FAQ

    We keep libraries for 1 month after sequencnig has occured to give you time to analyse your data. You can collect your libraries or we can safely dispose after 1 month.

    This is project specific but some yileds for popular workflows are:

    • For the polyA mRNA workflow we require 10ng-1ug of total RNA.
    • For Low Input mRNA workflow we require 2pg-200ng of total RNA.
    • For Total RNA-seq using rRNA depletion workflow we require 5ng-1ug of total RNA.
    • For Gene Expression Profiling RNA inputs of ≥200 ng are recommended to detect low abundant transcripts efficiently.
    • For Illumina DNA Prep with Exome 2.5 Plus Enrichment we require 50-1000ng high-quality genomic DNA. We have also shown this kit is compatible with FFPE DNA. This kit uses a TWIST Bioscience panel.

    Yes you can. Just be aware that you will need to pay VAT.

    Yes you can. Prepared libraries should be sent at ≥10 nM.  A volume of 15ul at the minimum is required. Concentration should ideally be determined using fluorometric means such as picogreen or Qubit. If you are pooling your libraries, the final pool should also be at ≥10 nM with a volume of at least 15ul.

     

    Yes you do. Please contact the team for the latest version of the sample submission forms at genetics_core_sequencing@mlist.is.ed.ac.uk

    This article was published on